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KMID : 0376219850220010143
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Chonnam Medical Journal
1985 Volume.22 No. 1 p.143 ~ p.161
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An Electron Microscopic Study on Fate of Tracer Material Absorbed by Epididymal Epithelium of Rat
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崔在權/Choi, Jae Kwon
朴誠植/金伯潤/安圭胤/李垠周/Park, Sung Sik/Kim, Balk Yoon/An, Kyu Youn/Lee, Eun Joo
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Abstract
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To investigate the fate of ferritin absorbed by canal epithelium in the tail portion of the rat epididymis, electron microscopic studies were made at various time intervals after ferritin administration. Ferritin was introduced into the epididymal lumen by a retrograde injection through the vas deferens until it seeped from the proximal incision. Tissues were examined 1, 2, 4, 6, and 8 weeks after ferritin administration. Results obtained were summarized as follows.
In 1 and 2 weeks after ferritin administration, ferritin was present only in vacuolar structures near the Golgi region of the principal cells. Clear cells had many vacuoles with homogenous density, but no or very few vacuoles contained ferritin. Halo cell, mainly observed in basal portion, contained a large amount of ferritin in the vacuoles of variable sizes and free ferritin in cytoplasm. In subepithelial layer, macrophages had a large amount of ferritin, and some smooth muscle cells also contained some amount of ferritin.
In 4 weeks after ferritin administration, in principal cells, vacuoles containing ferritin showed complex internal structures, and decreased in number having diminished amount of ferritin. Clear cells had no or few ferritin, but halo cells in epithelial layer and macrophages in subepithelial layer contained still large amount of ferritin.
In 6 and 8 weeks after ferritin administration, ferritin was still present in the vacuoles of the principal cells though, as a whole, the number of vacuoles containing ferritin were further decreased. In this period, halo cells could be categorized into three types: the first type had a large amount of ferritin in the giant vacuoles and free ferritin in cytoplasm, the second had ferritin in residual body like structures, and the third had a few vacuoles containing no ferritin.
These findings indicate ferritin absorbed by principal cells appeared to metabolized by the lysosomes and partly remained in the residual bodies for prolonged time period. Insignificant amount of ferritin noted within the clear cells may indicate either the rapid discharge of absorbed ferritin or rapid replacement of ferritin loaded cells with the newly grown clear cells. Halo cells were assumed to be macrophages migrated into epithelium from subepithelial layer and participated actively to remove ferritin in the epithelial layer as well as in the subepithelial tissue.
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